Composite
[OriTR]+[R
Part:BBa_K1439002
Designed by: Wenqi Li Group: iGEM14_OUC-China (2014-10-01)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation rate, conjugation time, and conjugation efficiency between different strains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1160
Illegal AgeI site found at 1272 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19
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Categories
Parameters
None |